Donald Trump signed an executive order on Wednesday banning transgender athletes from competing in women’s sports, reinforcing his administration’s broader rollback of transgender rights.

Axar.az, citing the Guardian, reports that titled “Keeping Men Out of Women’s Sports,” the order mandates immediate enforcement and directs federal agencies, including the Department of Justice, to interpret Title IX as prohibiting transgender women and girls from female sports categories. It also instructs state attorneys general to establish enforcement practices and threatens to cut federal funding for schools that allow trans athletes in female competitions.

The order coincided with National Girls and Women in Sports Day and is expected to face legal challenges. While the measure affects a small number of athletes—fewer than 10 trans athletes out of 520,000 in the NCAA—its implications are significant. The White House expects all sports bodies, including the NCAA, to comply with the directive.

LGBTQ+ advocacy groups condemned the order. Athlete Ally expressed heartbreak for trans youth, calling the policy a simplistic solution to a complex issue. Olivia Hunt, of Advocates for Trans Equality, described it as part of an ongoing attack on transgender rights.

This move follows a series of executive orders targeting trans rights. Trump recently banned gender-affirming care for individuals under 19 and signed another order reversing Biden-era policies on gender identity.